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KMID : 0903619940350040400
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Journal of the Korean Society for Horticultural Science
1994 Volume.35 No. 4 p.400 ~ p.405
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Influence of Sucrose , ABA and Daylength on Cormlet Formation of Gladiolus in Vitro
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Abstract
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This study was carried out to investigate the effects of sucrose, ABA, and daylength on cormlet formation from a shoot base of gladiolus cv. Topaz plantlet in vitro. Histological process during cormlet formation from the shoot base was also observed under light microscope.
In 9% sucrose concentration, cormlet formation was the best; the percentage of cormlet formation was 90%, the numbers of cormlets per shoot were 1.8, fresh weight per cormlet was 60.2 ㎎ and days required for cormlet formation was 59.1 days. With the addition of 10 μM ABA to 9% sucrose concentration, the days for cormlet formation was shortened to 46.1 days, but the percentage of cormlet formation, the numbers of cormlets per shoot, and fresh weight per cormlet was decreased. Light was necessary for forming cormlet from the shoot base of gladiolus in vitro. Daylength treatments were all effective, but the daylength over 16 hr was the most effective in the cormlet formation. There was positive correlation between cormlet´s diameter and weight; the trend was more pronounced in large cormlets over 4 ㎜ in diameter. In the results of histological observation, cormlet formation was began from node and internode at a shoot base and then developed to a complete globe-shaped cormlet. The vascular bundles were obvious in a mature cormlet and cells in a mature cormlet were larger compared to those of a immature cormlet. Appearance of the mature cormlet was similar to in vivo corn and the site of axillary buds in a cormlet were 1/2 alternate phyllotaxis.
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